Question

Suppose
have cloned
you
your gene .
of interest in
PET vector, which gives you
the provision to express
your protein in combination with hexa- Histidine-tag. .
Suitable for IMAC . After doing
After doing IMAC you
unable to find
your protein at the eluted fractions.
What could be the possible reasons and how can you
troubleshoot them?​

Answer 1

I DON'T GIVE LONG ANSWER

Answer:

MARK AS BRAINLIST