Question

Suppose the following base sequence was found in a 20-base DNA polymer,
3’ C-A-G-T-T-A-A-G-G-C-T-C-C-TA-G-G-T-T-A 5’

a. What would be the first five bases at the 3’ end of the complementary strand?
b. What would be the first 10 bases at the 5’ end of the complementary strand?
c. Assuming the presence of the complementary strand, what is the percentage composition of the polymer with respect to A-T base pair and with respect to the G-C base pair?​

Answer 1

Answer: (a) 3' T-A-A-C-C 5'

(b) 5' G-T-C-A-A-T-T-C-C-G 3'

(c) the percentage composition of the polymer with respect to A-T base pair and with respect to the G-C base pair is 40%.

Answer 2

a. 3' T-A-A-C-C 5'

b.5' G-T-C-A-A-T-T-C-C-G 3'

c.the percentage composition of the polymer with respect to A-T base pair and with respect to the G-C base pair is 40%

Explanation:

1.Complementary DNA (cDNA) is a copy of a region of a strand of DNA.

2.For example, if the original DNA stand had a sequence of ATT, the complementary sequence will be TAA. The cDNA will bind to the complementary site on the DNA strand.

3.DNA polymerase adds nucleotides to the three prime (3')-end of a DNA strand, one nucleotide at a time.

4.Every time a cell divides, DNA polymerases are required to duplicate the cell's DNA, so that a copy of the original DNA molecule can be passed to each daughter cell.