Suppose you have a DNA solution that is 200 ng/μl, and you want to use 0.05 mg of DNA in a
reaction. How many microliters of your DNA solution should you use?
Answers
Explanation:
2.84 ng/ul X 5244 ul = 14893 ng = 14.8 ug - correct.
Everything you need for all conversions is the formula relating amount (N), volume (V) and concentration (C):
C = N / V
(concentration is amount per volume) You solved this by N, getting N = C x V, to calculate the total amount of the solution with given C=2.84ng/ul in a volume of V=5422ul.
Your concentration (2.84 ng/ul) is smaller than the minimum concentration required by the centre (5ng/ul). So you will need to concentrate the samples (speed-vac, reduce the volume to less than 50%).
Given you want 50ul with a conc of 10ng/ul (twice the minimum required conc), this is how you can proceed:
The desired amount is N = C x V = 10ng/ul x 50ul = 500ng.
This amount is contained in V = N / C = 500ng / 2.84ng/ul = 176ul of your sample.
Take this volume of your sample and concentrated it in a speed-vac to a volumen of less than 50ul.
Measure the obtained volume of the concentrated sample (good enough to use a pipet for this purpose) and adjust the volume to 50ul by adding water or buffer.
Done. plz thank me