Question

Surface sterilization of explant were carried by using what?

Answer 1

Answer:

Surface sterilization with 70% ethanol for 1 min followed by 1% sodium hypochlorite (NaOCI) (+ 2-3 drops of Tween 20) for 15 min proved most effective for maximum survival percentage (99%) in leaf explants whilst 70% ethanol washing for 2 min, followed by 0.1% mercuric chloride (HgCl2) for 5 min proved to be more ...

Answer 2

Answer:

Surface sterilization with 70% ethanol for 1 min followed by 1% sodium hypochlorite (NaOCI) (+ 2-3 drops of Tween 20) for 15 min proved most effective for maximum survival percentage (99%) in leaf explants whilst 70% ethanol washing for 2 min, followed by 0.1% mercuric chloride (HgCl2) for 5 min proved to be more ...