Question

Synthesis of simple peptides by n protection and c protection

Answer 1

From the perspective of an organic chemist, peptide synthesis requires selective acylation of a free amine. ... Consequently, the acylation of amino acids by treatment with acyl chlorides or anhydrides at pH > 10, as described earlier, serves to protect their amino groups from further reaction.

Answer 2

Unlike ribosome protein synthesis, artificial synthesis builds peptides in the C to N direction. During solid-phase peptide synthesis, each peptide is anchored to an insoluble polymer at the C-terminus. The free N-terminal amine is coupled to a single N-protected amino acid unit.