Question

tell me the process of testing proteins in groundnuts

Answer 1

Detection of Carbohydrates, Proteins and Fats

Theory

Real Lab ProcedureTest for CarbohydratesTest For GlucoseBenedict's TestMaterials Required:

Procedure:Using a dropper, take a small quantity of Benedict’s reagent.Add the reagent to the test tube containing banana extract.Boil the sample over a burner for 2 minutes holding the test tube firmly with a test tube holder.Keep shaking the test tube as it is being heated.A brick red precipitate appears, indicating the presence of glucose in the banana extract.Conclusion:

On boiling banana extract with the Benedict’s reagent, the cupric ion present in the Benedict’s reagent is reduced by the reducing agent, sugar, to form a brick red coloured precipitate of cuprous oxide.

Fehling's TestMaterials Required:

Procedure:Using a dropper, take a small quantity of Fehling’s solution A.Add the Fehling’s solution A to the test tube containing banana extract.Using a dropper, take a small quantity of Fehling’s solution B.Add Fehling’s solution B to the test tube containing banana extract.Boil the sample over a burner for 2 minutes, holding the test tube firmly with a test tube holder.Keep shaking the test tube while heating.A brick red precipitate appears, indicating the presence of glucose in the banana extract.Conclusion:

The cupric ion present in the Fehling’s solution is reduced on boiling by the reducing substance, sugar, to form the brick red coloured precipitate of cuprous oxide.

Test for SucroseMaterials Required:

Procedure:Using a dropper, take a small quantity of concentrated HCl.Add 2 to 3 drops of concentrated HCl to the test tube containing sugarcane extract.Boil the sample over a burner for 2 minutes, holding the test tube firmly with a test tube holder.This hydrolyses sucrose into glucose and fructose.Using a dropper, take a small quantity of NaOH solution.Add few drops of NaOH solution to the test tube to make the solution alkaline.Now we can perform Benedict’s test to this solution to test for the presence of glucose.Using a dropper, take a small quantity of Benedict’s reagent.Add the reagent to the test tube containing the sample.Boil the sample over a burner for 2 minutes, holding the test tube firmly with a test tube holder.The colour changes from blue to green and finally to orange or brick red, indicating the presence of glucose.Test for StarchMaterials Required

ProcedureUsing a dropper, take a small quantity of iodine solution.Add 5 drops of iodine solution to the test tube containing potato extract.Blue black colour indicates the presence of starch in potato extract.Test for ProteinsBiuret TestMaterials Required

ProcedureUsing a dropper, take a small quantity of 40% NaOH solution.Add a few drops of NaOH solution to the test tube containing egg albumin.Using a dropper, take a small quantity of 1% CuSO4 solution.Add 2-3 drops of CuSO4 solution to the test tube containing egg albumin.Shake the solution to mix it well.A violet colour appears in the test tube, which indicates the presence of proteins.Xanthoproteic TestMaterials required

ProcedureUsing a dropper, take a small quantity of concentrated HNO3.Add 5 drops of Concentrated HNO3 to the test tube containing egg albumin.Boil the sample over a burner for 2 minutes, holding the test tube firmly with a test tube holder. Yellow precipitate appears in the test tube.Using a dropper, take a small quantity of ammonia solution.Add a few drops of ammonia solution to the sample.Shake the solution to mix it well.Yellow ppt. changes to orange in colour, which indicates the presence of protein.Million’s TestMaterials Required

ProcedureUsing a dropper, take a small quantity of Million's regent.Add few drops of Million's reagent to the test tube containing egg albumin.Wait for some time.Pink colour appears in the test tube, which indicates the presence of protein.Test for FatsSudan III TestMaterials Required:

Procedure:Using a dropper, take a small quantity of Sudan III reagent.Add few drops of Sudan III reagent to the test tube containing egg albumin.Shake the solution to mix it well.Pink droplets appear indicating the presence of fat in the sample.Paper Spot TestMaterials Required:

Peanut seeds and piece of white paper.

Procedure:Take a peanut seed from the watch glass.Crush the peanut seed and rub it on a piece of white paper.Paper becomes translucent at the spot, which indicates the presence of fat.Simulator Procedure (as performed through the Online Labs)

You can select the type of test from the ‘Select the food substance’ drop down list.

Carbohydrates

You can select the type of test from the ‘Select the carbohydrate’ drop down list.

Glucose

You can select the type of test from the ‘Select

Answer 2

Answer:

Explanation:

The process of testing proteins in groundnuts:

Procedure:

To perform a simple test to identify the presence of proteins

Materials Required

• Stirrer

• Dropper

• Water bath

• Distilled water

• Bunsen burner

• Test tube holder

• Clean and dried test tubes

• Food sample – gound nuts.

Chemical required:

Copper sulfate solution.

Biuret reagent solution or solution of copper sulfate and caustic soda.

Procedure:

Biuret Test:

• Take a cleaned and dried test tube.

• Add the gound nut sample into the test tubes.

• Add 2ml of sodium hydroxide and 5 to 6 drops of copper sulfate solution to it.

• Shake the test tube gently to mix the ingredients thoroughly and allow the mixture to stand for 4 – 5 minutes.

• If there is the appearance of bluish- violet color, it indicates the presence of protein.

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