Question

$\bold\red\underline{what\: is\: chromatography}$??​

Answer 1

Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation.

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Answer 2

Chromatography is a technique which is used in the pharmaceutical field for the separation of mixtures. In which separation of the mixture into their component in the order to firstly analyze then identify and finally purify the component or mixture.

Definition:

Chromatography is defined as technique or process in which a chemical mixture carried by liquid or gas is separated into the different component as a result of differential distribution of the solute as they flow around a stationary solid or liquid phase

The principal of chromatography

Chromatography is consists of two different phase. One is a mobile phase and second is a stationary phase. The mobile phase contains a mixture of two or more solvents. In which volatile solution are mostly used. This volatile solution mixture takes into the beaker and pack by watch glass and any other suitable items. Then took it for 30 minutes

The stationary phase is either paper or glass slide having silica gel for stationary phase are using. And the interaction between the mobile phase and the stationary phase result in the separation of a component from the mixture this result show on the slide or paper. Mixture the length of compound line on paper in cm.

Applications of chromatography

The chromatography techniques are mostly used in the separation process of carbohydrates, amino acids, and proteins.

2. It is very helpful for the analysis of the qualitative and quantitative purpose of the complex sample mixture.

3. The process is also used in the determination of proteins molecular weight.

4. Chromatography is also useful in the analysis process of drugs, vitamins, and hormones.

Types

1. Affinity chromatography

2. Gel Filtration Chromatography

3. Column Chromatography

4. Thin Layer Chromatography

5. Paper Chromatography

6. Gel Chromatography

7. Ion Exchange Chromatography

8. Gas-Liquid Chromatography

9. HPLC High-Performance Liquid Chromatography.