Question

$\huge \bf \underline \green{question}$

ᴅɪꜰꜰᴇʀᴇɴᴛɪᴀᴛᴇ ʙᴇᴛᴡᴇᴇɴ ɪꜱᴏᴇʟᴇᴄᴛʀɪᴄ ᴘʀᴇᴄɪᴘɪᴛᴀᴛɪᴏɴ ᴀɴᴅ ɪꜱᴏᴇʟᴇᴄᴛʀɪᴄ ꜰᴏᴄᴜꜱꜱɪɴɢ
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Explanation needed✅​

Answer 1

ᴀɴsᴡᴇʀ-:

ɪsᴏᴇʟᴇᴄᴛʀɪᴄ ᴘʀᴇᴄɪᴘɪᴛᴀᴛɪᴏɴ-:

⇨4.6 Isoelectric Point Precipitation

The isoelectric point (pI) is the pH of a solution at which the net charge of a protein becomes zero. At solution pH that is above the pI, the surface of the protein is predominantly negatively charged, and therefore like-charged molecules will exhibit repulsive forces.

ɪsᴏᴇʟᴇᴄᴛʀɪᴄ ғᴏᴄᴜssɪɴɢ-:

⇨IEF, also known simply as electrofocusing, is a technique for separating charged molecules, usually proteins or peptides, on the basis of their isoelectric point (pI), i.e., the pH at which the molecule has no charge. IEF works because in an electric field molecules in a pH gradient will migrate towards their pI.

ʙᴇ ʙʀᴀɪɴʟʏ.ツ

Answer 2

$\huge\tt\underline\pink{answer}$

$\tt\underline\blue{isoelectric \: precipitation}$

•The isoelectric point (pI) is the pH of a solution at which the net charge of a protein becomes zero. At solution pH that is above the pI, the surface of the protein is predominantly negatively charged, and therefore like-charged molecules will exhibit repulsive forces.

$\tt\underline\green{isoelectric \: foccusing}$

•Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different molecules by differences in their isoelectric point (pI). It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest is a function of the pH of its surroundings.

@itzẞparkly!♥️