Question

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What is tissue culture?
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Answer 1

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Tissue culture is the artificial method of reproduction where a small cutting of plant such as root or stem is taken and is grown in laboratory condition by providing it with suitable nutrients in a media and growing them in a petri plate or test tubes. The plant is provided with the most hygienic condition.

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Answer 2

Answer:

This chapter describes the use of pronase in tissue culture. Pronase, an enzyme preparation from Streptomyces griseus, consists of a mixture of neutral and alkaline proteases, aminopeptidases, and carboxypeptidases. It has been used to remove the zona pellucida of mammalian eggs, to liberate chondrocytes from cartilage prior to freezing, and in tissue culture. Applications of Pronase in tissue culture include the dissociation of chicken and mouse embryo cells prior to cultivation and subcultivation. Pronase acts more rapidly than trypsin.