Question

The action of alcohol during Gram
staining is
O Allows the color
O It adds color
O Decolorises the cells
O None of these​

Answer 1

Answer:

The alcohol dissolves lipids found in the outer cell membrane of Gram-negative bacteria, allowing the crystal violet-iodine complex to leak out of the thinner peptidoglycan layer. The alcohol is added for 10 to 20 seconds; it is poured on the slide until all the iodine is washed away and the run-off is colorless