Question

The action of alcohol during gramstaining is

Answer 1

Answer:

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Either acetone or ethyl alcohol can be used as the decolorizing agent. The alcohol dissolves lipids found in the outer cell membrane of Gram-negative bacteria, allowing the crystal violet-iodine complex to leak out of the thinner peptidoglycan layer. The alcohol is added for 10 to 20 seconds; it is poured on the slide until all the iodine is washed away and the run-off is colorless. At this point in the Gram stain process, Gram-negative bacteria are colorless while Gram-positive bacteria still retain the crystal violet. Once finished the slide needs to be rinsed with water to stop the decolorizing effect.

Answer 2

Answer:

The alcohol dissolves lipids found in the outer cell membrane of Gram-negative bacteria, allowing the crystal violet-iodine complex to leak out of the thinner peptidoglycan layer. The alcohol is added for 10 to 20 seconds; it is poured on the slide until all the iodine is washed away and the run-off is colorless.