The activity of Enzyme X (total volume 5.3 ml) is 2.34 micromoles of product formed per min. The total protein content of this solution is 0.8 mg. What is the specific activity?
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Specific activity formula
Therefore, specific activity is calculated by dividing the number of units/mL by the protein concentration in mg/mL to get μmol/min/mg. For example: The specific activity of the isolated enzyme was measured at 150 μmoles/min/mg protein before purification and 800 μmoles/min/mg, after purification.
Higher Classifications: Protein
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