Question

The black rods in this image are single-celled organisms found in a pond.
Which scientist was the most likely to observe this organism under the microscope first?

Answer 1

Answer:

Antonie Philips van Leeuwenhoek[note 2] FRS (/ˈɑːntəni vɑːn ˈleɪvənhuːk, -hʊk/ AHN-tə-nee vahn LAY-vən-hook, -⁠huuk; Dutch: [ɑnˈtoːni vɑn ˈleːuə(n)ˌɦuk] (About this soundlisten);[5] 24 October 1632 – 26 August 1723) was a Dutch businessman and scientist in the Golden Age of Dutch science and technology. A largely self-taught man in science, he is commonly known as "the Father of Microbiology", and one of the first microscopists and microbiologists.[6][7] Van Leeuwenhoek is best known for his pioneering work in microscopy and for his contributions toward the establishment of microbiology as a scientific discipline.

Answer 2

Explanation:

Direct observation allows the viewing of the living organisms as they move around.

For direct observation, a sample of water can be directly observed under a microscope, or the organisms can be cultured to increase the number before the inspection.

Under direct observation, Amoeba appears like a transparent jelly-like structure that shows the crawling movement of the organism through the field.

Pseudopodia can be observed as the cell membrane protrudes, forming long finger-like projections.

Inside the organism vacuoles are seen as large empty spaces and food particles are seen as tiny black dots.

Observation after staining

In order to observe the internal cell organelles of the organism, fixing and staining procedures are performed.

Fixing kills the organisms and thus isn’t useful to observe the motility of the organism.

However, fixing and staining provide a better understanding of the structure and morphology of the organism.

After staining the organism, it can be observed that Amoeba’s cell organelles and cytoplasm are enclosed inside the cell membrane.

The cytoplasm is stained, which allows the observation of food vacuoles, nucleus, and other essential cell organelles.

Amoeba usually has one or more nucleus, which is present inside the nuclear membrane.

Food particles can be seen present inside vacuoles where they are stored and digested.

Similarly, to maintain the osmotic balance, contractile vacuole can also be seen throughout the cytoplasm.