The cell-free extract prepared from E. coli cells over-expressing enzyme β-glucosidase showed the activity of 1.5 units per ml (protein concentration 2 mg per ml). The Ni-NTA purified preparation showed the activity of 75 units per ml (protein concentration 100 μg per ml). Calculate the fold purification of the enzyme achieved?
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थॅबथॅडौऋडौडथंओजौऐडौओडौऐडैऔडैऔजजौऐ
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