Question

The chromatographie peaks for analytes X and Y
are found to have retention times of 16.20 and 17.93
min, respectively, on a 30 cm column. Unretained
species is found to have retention time of 1.30 min.
The base widths of peaks X and Y are 10 min and
1.21 min, respectvoly Calculate the average
number of theoretical plates N, plate holgint H for
the column and separation factor a for X and Y.​

Answer 1

The chromatographie peaks for analytes X and Y

are found to have retention times of 16.20 and 17.93

min, respectively, on a 30 cm column. Unretained

species is found to have retention time of 1.30 min.

The base widths of peaks X and Y are 10 min and

1.21 min, respectvoly Calculate the average

number of theoretical plates N, plate holgint H for

the column and separation factor a for X and Y.