The coding sequence of a gene xlr18 has the single orf of 783 bp. The approximate molecular weight of the xlr18 protein in kda is
Answers
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Given - ORF - 783 bp
Find - Molecular weight of protein
Solution - The stated ORF contains 783 base pairs. We know 3 bases, which are known as codon, code for single amino acid. Joining of amino acid gives proteins.
In normal codes, first three are start codon which can translate and three stop codon which do not code for amino acid.
Therefore, considerable number of bases will be 780.
Since 3 codon make 1 amino acid, hence 780 codon will make - 260 amino acids.
Combination of 260 amino acid will make a protein. One amino acid has average molecular weight of 110 Da.
Hence, protein will weigh - 110*260
- 28,600 Da = 28.6 KDa.