Question

The colorimetric detection methods normally involve biochemical reactions (chromophore development) whereas the fluorescent/ luminescent based detection systems offer straightforward quantitations using the Multilabel reader, Victor X3. Briefly explain the use of multilabel reader for the analysis of:
a. Green Fluorescent protein analysis
b. Luciferase detection

Answer 1

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.[2][3] Similar proteins that also fluoresce green are found in many marine organisms, but the label GFP traditionally refers to this particular protein, which was first isolated from the jellyfish Aequorea victoria and is sometimes called—when such precision is required—avGFP.

Explanation:

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