Question

the common locating agent used to identify amino acid is​

Answer 1

Answer:

Ninhydrin

Explanation:

Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [ due to the formation of the complex - Rheuman's purple] . Rf values can be calculated and compared with the reference values to identify the amino acids. [The Rf value for each known compound should remain the same provided the development of plate is done with the same solvent, type of TLC plates, method of spotting and in exactly the same conditions].

Answer 2

Answer:

Ninhydrin

Explanation:

Since amino acids are colourless compounds, ninhydrin is used for detecting them.