Question

The conc. of yeast t-RNA in
aqueous solution in 10 M The absorbance is found to be 0.209 when the
soln is placed in 1.00 um cuvette and 258nm radiations
are passed through it
> Calculate the specific absorptivity, including units of yeast t-rna
what will be the absorbance if the solution is 5M
what will be the absorbance it the path length
of original solution is
to 5.00 cm?
​

Answer 1

Answer:

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Answer 2

The value of specific absorptivity is 47.84.

Step-by-step explanation

Given:

The concentration of yeast solution = 10M

Absorbance = 0.209

The length of the cuvette 1.00 um

To find = specific absorptivity

Solution:

We can use the formula for absorbance:

a = εcl

a = absorbance,

ε= specific absorptivity

c= concentration,

l = length

Putting, the values, we get:

0.209 = ε×10× 1

ε = 10× 1 ÷ 0.209

ε = 47.84

Now, we are given the solution's concentration as 5M.

Absorbance  = εcl

A=  47.84×5×1

A = 239.2

Now, we need to calculate the absorbance when the length of the cuvette is 5 cm.

so, A = 47.84×10×5

A= 239.2

Result:

Thus, the value of specific absorptivity is 47.84.

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