The diagnostic value of determining the activity of aminotransferases in the blood . Give examples
Answers
Answer:
The transaminase enzymes are important in the production of various amino acids, and measuring the concentrations of various transaminases in the blood is important in the diagnosing and tracking many diseases. For example, the presence of elevated transaminases can be an indicator of liver and cardiac damage.
Answer:
Aminotransferases or transaminases are a group of enzymes that catalyze the interconversion of amino acids and oxoacids by transfer of amino groups. Aspartate aminotransferase (AST), formerly termed glutamate oxaloacetate transaminase (GOT), and alanine aminotransferase (ALT), formerly termed glutamate pyruvate transaminase (GPT), are the two aminotransferases of greatest clinical significance. Pyridoxal-5′-phosphate (P5′P) functions as coenzyme in the amino transfer reactions. In all amino transfer reactions, 2-oxoglutarate and l-glutamate serve as one amino group acceptor and donor pair.
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The specificity of individual enzymes determines the specific amino acid that serves as the other amino group donor. In the AST reaction, the l-amino acid is aspartate; for ALT, it is alanine. The reactions are shown on the facing page
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