Question

The different steps of recombinant DNA technology are given below randomly.
(i) Isolation of the DNA fragments or genes to be cloned
(ii) Introducting of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation)
(iii) Multiplication/expression of the introduced gene in the host
(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment
(v) Insertion of the isolated gene in a suitable plasmid vector
Which of the following represents the correct sequences of steps ?

Answer 1

Answer:

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Steps of recombinant DNA technology;

Isolation of genetic material i.e; isolation of the DNA fragments or genes to be cloned

Restriction enzyme digestion

Amplification

Ligation

Insertion of recombinant DNA into host i.e; insertion of the isolated gene in a suitable plasmid vector.

Transformation i.e; Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation).

Obtaining foreign gene product i.e; multiplication/expression of the introduced gene in the host.

Down streaming process i.e; Selection of the transformed host cells and identification of the clone containing the desired gene/DNA fragment.

So the correct Answer is (i),(v),(ii),(iv),(iii)

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Answer 2

Answer:

correct Answer is (i),(v),(ii),(iv),(iii)

• Isolation of the DNA fragments or genes to be cloned.
• Introducting of the recombinant DNA into a suitable cell (usually E. coil) called host (transformation).
• Multiplication/expression of the introduced gene in the host.
• Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment.
• Insertion of the isolated gene in a suitable plasmid vector.

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