The distance on chromatogram from the
point of injection of sample to the center of
peak is called
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The mobile phase and injected sample mixture enters the column, passes through the ... The center of the band contains the highest concentration of analyte ...
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The retention distance is the distance between the injection point and the recorder's greatest peak.
- The graph depicts the relationship between time and solute concentration.
- Two components of the concentration profile impact a column's effectiveness and, as a result, its capacity to separate or resolve solute zones.
- The first is peak maximum, which is the highest concentration point of a peak.
- To attain acceptable resolution, two adjacent peaks' maxima must be decoupled.
- Disengagement is influenced by the solute's identification as well as the selectivity of the stationary and mobile phases.
- The second factor that influences efficiency and resolution is the peak's width.
- Peaks with widely separated maxima may be too broad to resolve the solutes. As a result, in chromatography, peak width is an important factor.
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