the effect orthosilicic acid on collagen type i, alkaline phosphatase and osteocalcin mrna expression in human bone-derived osteoblasts in vitro. Bioceramics.,
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The object of this study was to investigate the effect of the concentration of orthosilicic acid (0, 0.5, 1, 5 and 10μM) on gene expression in human osteoblast cells isolated from trabecular bone. This was measured using reverse transcriptase-polymerase chain reaction (RT-PCR) to quantify messenger RNA (mRNA) levels for collagen type I, alkaline phosphatase and osteocalcin. Results showed that while collagen type I mRNA expression was increased by the addition of up to 10μM orthosilicic acid, ALP message was suppressed over time and osteocalcin levels were decreased.
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