The experiment,” to prove that carbon dioxide is produced by green plants during respiration” is carried out in the dark. Why?
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Put some germinating seeds in a conical flask.
The conical flask is fitted to a delivery tube bent twice at right angles by means of cork.
Suspend a small test tube containing 20% potassium hydroxide.
Dip the free end of the bent tube in a beaker of coloured water.
Leave it for a day. Note the changes.
The germinating seeds respires aerobically by utilizing the oxygen in the flask.
Carbon dioxide released during the aerobic respiration is absorbed by potassium hydroxide and creates a vacuum and causes rise of water in the bent tube.
If we put a burning candle in the setup, the candle extinguishes which proves oxygen is utilized by germinating seed for respiration.
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