The factor affecting separation of compound in the gas chromatography
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Which factors influence the separation of the components? The boiling point of a compound is often related to its polarity (see also polarity chapter). ... As a result, polar compounds have long retention times on polar stationary phases and shorter retention times on non-polar columns using the same temperature.
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The best separations are usually observed for temperature gradients, because the differences in polarity and in boiling points are used here. A high flow rate reduces retention times, but a poor separation would be observed as well.
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