The failure of oncometabolites to adapt the type 2 diabetic heart and kidneys to hypoxia
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Nearly two thirds of all deaths associated with type 2 diabetes are from heart and kidney. Ischaemic damage, where there is a reduction in oxygen (hypoxia) and substrates, is common in diabetic heart and kidney disease. The aim of this PhD is to develop cellular models of insulin resistance in cardiac and renal cells. The candidate will use a combination of molecular biology tools, including western blotting, qPCR, fluorescence imaging, and fluorescent plate based assays, along with pyrosequencing. These techniques will be used to characterise the role that oncometabolites (L2HG, succinate and fumarate) play in regulating key DNA and histone methylation target genes (epigenetic markers) in their insulin resistant models.
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Nearly two thirds of all deaths associated with type 2 diabetes are from heart and kidney. Ischaemic damage, where there is a reduction in oxygen (hypoxia) and substrates, is common in diabetic heart and kidney disease. The aim of this PhD is to develop cellular models of insulin resistance in cardiac and renal cells. The candidate will use a combination of molecular biology tools, including western blotting, qPCR, fluorescence imaging, and fluorescent plate based assays, along with pyrosequencing. These techniques will be used to characterise the role that oncometabolites (L2HG, succinate and fumarate) play in regulating key DNA and histone methylation target genes (epigenetic markers) in their insulin resistant models.
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