The GOD-POD method does NOT require a. Sulfuric Acid b. Amylase c. Glucose Oxidase d. Peroxidase.
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Answer:
Option:D.Peroxidase
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The correct option is d. Peroxidase. The GOD-POD method is a commonly used method for measuring glucose levels in biological samples. This method uses only glucose oxidase, and not peroxidase.
The principle of the method is based on the oxidation of glucose by glucose oxidase to produce gluconic acid and hydrogen peroxide. The hydrogen peroxide produced is not further converted by peroxidase. Instead, the change in the concentration of hydrogen peroxide can be measured spectrophotometrically to determine the glucose concentration in the sample.
This method is a simple, reliable, and cost-effective way to measure glucose levels in various types of samples, including serum, plasma, urine, and food. It does not require the use of additional enzymes, such as amylase, or chemicals, such as sulfuric acid, which are typically used in other glucose determination methods.
In conclusion, the GOD-POD method only requires glucose oxidase and does not require peroxidase. This method is commonly used in clinical and research settings due to its simplicity, reliability, and cost-effectiveness, making it an important tool for glucose determination.
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