The main advantage of fluorescence over uv-visible spectroscopy is
a) its sensitivity
b) its compatibility with separation technique
c) its compatibility with most of the analytes
d) none of the above
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c
it's comapatibility with most of the analytes
it's comapatibility with most of the analytes
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The main advantage of fluorescence over UV-visible spectroscopy is (A) its sensitivity.
Explanation:
- Compared to ultraviolet-visible absorption measurements, fluorescence spectroscopy has various advantages. Its extremely low detection limit is a significant advantage.
- While most molecules absorb at some wavelength, it is limited to the small number of molecules that fluoresce or can be induced to glow.
- Low amounts of light are very easy to detect, but minor variations in intensity are difficult to distinguish.
- Fluorescence spectroscopy is thus more sensitive than UV-Visible absorption spectroscopy.
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