Question

The main advantage of fluorescence over uv-visible spectroscopy is



a) its sensitivity



b) its compatibility with separation technique



c) its compatibility with most of the analytes



d) none of the above

Answer 1

c
it's comapatibility with most of the analytes

Answer 2

The main advantage of fluorescence over UV-visible spectroscopy is (A) its sensitivity.

Explanation:

• Compared to ultraviolet-visible absorption measurements, fluorescence spectroscopy has various advantages. Its extremely low detection limit is a significant advantage.
• While most molecules absorb at some wavelength, it is limited to the small number of molecules that fluoresce or can be induced to glow.
• Low amounts of light are very easy to detect, but minor variations in intensity are difficult to distinguish.
• Fluorescence spectroscopy is thus more sensitive than UV-Visible absorption spectroscopy.