The Michaelis-Menten and the Lineweaver-Burk plots can be used to determine
the Km and Vmax of the enzyme-catalysed reaction. Explain the significance of
Km value in enzyme catalysed reaction.
By using succinate dehydrogenase as the enzyme, sketch a Lineweaver-Burk plot
that shows an enzyme catalysed with and without competitive inhibitor. Explain
what will happen if malonate is added to the reaction. Relate the Lineweaver-Burk
plot to the effect of malonate on Km and Vmax values.
Using the knowledge from cell biology, explain how the enzyme is being
synthesis.
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How do you find the Km and Vmax of a Michaelis Menten graph?
From the graph find the maximum velocity and half it i.e. Vmax/2. Draw a horizontal line from this point till you find the point on the graph that corresponds to it and read off the substrate concentration at that point. This will give the value of Km.
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