The milestone that defines anaphase does when the chromosomes are chromatics
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In metaphase all the chromatin fibre gets condensed resulting in the formation of chromosome which are visible in under the microscope
Hence chromosome gets arranged in the straight line
In metaphase the chromosome which are arranged at the center gets spilt resulting in the formation of two daughter chromosome.
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in anaphase the chromosomes move apart due to division of centromere as a result two daughter chromosomes from each pair due to contraction of spindle fibre
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