Question

the number of fragments genrated by the protease cleavage of an antibody is​

Answer 1

Answer:

Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme. Papain cleaves immunoglobulin G molecules in the hinge reason which results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain.

Answer 2

Answer:

Antibodies are powerful tools for protein and molecular detection and purification. Although whole antibodies (usually IgG or IgM) are ideal for most immunoassay applications, the performances of certain procedures are enhanced by using antibody fragments, such as Fab and F(ab')2. This article reviews the benefits, types and methods for preparing antibody fragments.