The pcr (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called thermus aquaticus in a hot spring inside yellowstone national park, wyoming. This organism contains a heat-stable form of dna polymerase known as taq polymerase, which continues to function even after it has been heated to 95 degrees
c. Why would such a heat-stable polymerase be beneficial in pcr?
Answers
Answer:
Hot-start PCR prevents the amplification of non-specific products, amplifies low abundance targets and offers convenient room temperature reaction setup. We offer different hot-start DNA polymerases to support your everyday research needs. This includes Thermo Scientific DreamTaq Hot Start DNA Polymerase, which is an enhanced hot start Taq DNA polymerase suitable for most PCR applications. DreamTaq Hot Start offers higher sensitivity, specificity, and yields compared to conventional hot start Taq DNA polymerase.
Answer:
The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat-stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been heated to 95°C. Why would such a heat-stable polymerase be beneficial in PCR?
A) Each cycle includes a "hot" denaturation phase (95°C), which separates the hydrogen
bonds that hold the strands of the template DNA together.
B) Each cycle includes a "hot" denaturation phase (95°C), which serves to sterilize the
culture.
C)Each cycle includes a "hot" denaturation phase (95°C), which activates the Taq
polymerase.
D)Each cycle includes a "hot" saturation phase (95°C), which allows the primers to
anneal to the target DNA.
E)More than one of the above are correct.