Biology, asked by doctorsunidhi8790, 1 year ago

The principles of growing of microbial and animal cells

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Answered by omkalamkar9999
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Nine amino acids, referred to as the essential amino acids, cannot be synthesized by adult vertebrate animals and thus must be obtained from their diet. Animal cells grown in culture also must be supplied with these nine amino acids, namely, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine. In addition, most cultured cells require cysteine, glutamine, and tyrosine. In the intact animal, these three amino acids are synthesized by specialized cells; for example, liver cells make tyrosine from phenylalanine, and both liver and kidney cells can make glutamine. Animal cells both within the organism and in culture can synthesize the 8 remaining amino acids; thus these amino acids need not be present in the diet or culture medium. The other essential components of a medium for culturing animal cells are vitamins, which the cells cannot make at all or in adequate amounts; various salts; glucose; and serum, the noncellular part of the blood.

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