The protein concentration and enzyme activity in 100 mL of a cell free extract is 5 mg/mL and 2
units/mL, respectively. After multiple steps of purification, the final 10 mL fraction contains 4 mg/mL
of protein and 15 units/mL of enzyme activity. The fold purification and percentage recovery,
respectively is:
Answers :
1. 10 and 75
2. 9.4 and 20
3. 9.4 and 75
4. 10 and 20
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