The ratio between number of ester bonds
formed and new hydrogen bonds formed to
form recombinant DNA by using ECO RI
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In molecular biology it is used as a restriction enzyme. EcoRI creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G/AATTC, which has a palindromic, complementary sequence of CTTAA/G.
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