Question

The reactions occur in tris HCl buffer

Answer 1

Answer:

Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. ... A Tris buffer solution can be made by mixing Tris with Tris-HCl. This prevents overshooting the pH and prevents the need to work with strong acids or bases.