Question

The restriction site of EcoRI is GAATTC. If a random sequence of genomic dna is cleaved with
this restriction enzyme, what will be the average size of the frangement prodeced at GC
content of the genomic DNA is 40%?​

Answer 1

Answer:

EcoRI ligation. The restriction endonuclease enzyme EcoRI recognizes the ssDNA sequence 5'-GAATTC'-3, and introduces a single-strand cut between the G & A nucleotides. This recognition site is a palindrome: the opposite strand also reads 5'-GAATTC'-3 and will be cut in the same manner.