Question

The results of gel electrophoresis are shown below. What can you determine about the DNA from looking at the results of this test?

Answer 1

Gel electrophoresis is a technique used to separate DNA fragments according to their size.

DNA samples are loaded into wells (indentations) at one end of a gel, and an electric current is applied to pull them through the gel.

DNA fragments are negatively charged, so they move towards the positive electrode. Because all DNA fragments have the same amount of charge per mass, small fragments move through the gel faster than large ones.

When a gel is stained with a DNA-binding dye, the DNA fragments can be seen as bands, each representing a group of same-sized DNA fragments.

Introduction

Suppose you have just done a PCR reaction, making many copies of a target DNA region. Or perhaps you’ve done some DNA cloning, trying to "paste" a gene into a circular DNA plasmid.

Answer 2

Uses of Gel Electrophoresis

Explanation:

• Gel electrophoresis is a method used to isolate DNA parts as indicated by their size
• DNA samples are loaded into wells (spaces) toward one side of a gel, and an electric current is applied to get them through the gel
• Electrophoresis analysis is utilized in forensics sciences to look at DNA, in clinical research facilities to do genetic testing, and in microbiology labs to recognize microorganisms
• It is a technique utilized for separation of substances of different ionic properties on electric field, DNA parts are - ive charged atoms advances toward anode as indicated by their molecular size through agrose gel