The science of getting exact copies of desired living organism is known as
Answers
Answered by
0
cloning in genetic from which exact copy us produced ........ may be the answer of your question
Answered by
0
Answer:
The science of getting exact copies of desired living organisms is known as cloning.
Explanation:
The process in which a gene of interest is located and copied of all the DNA extracted from an organism.
It is a widely applied technique in the field of DNA and protein research as well as in the field of molecular biology. Various methods are used for gene cloning.
They are:
- Restriction enzyme digestion and ligation.
- Transformation
- Recombinase dependent cloning
- PCR cloning
- TA cloning
- Ligation independent cloning
Basically, gene cloning involves the following steps:
- The gene of interest is that organism's DNA is extracted and cut into the size of genes with the help of restriction enzymes.
- The bacterial plasmids (DNA) are also cut with the same enzyme.
- Both cut DNA and plasmid are kept together in a test tube. Their combination gives rise to recombinant DNA.
- The recombinant DNA is transferred into bacteria using heat shock.
- The bacteria multiply into colonies called gene libraries.
- The gene library is then screened to identify the gene of interest by observing three things on each i.e a sequence of DNA similar to the gene of interest, a protein encoded by a gene of interest, a DNA marker with a location close to a gene of interest.
Similar questions