The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to be 2.7 x 106 cells/ml. The culture is diluted 1:27 and then 100μl seeded per well into a 96 well plate. What is the final cell density per well?
Answers
Explanation:
The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to be 2.7 x 106 cells/ml. The culture is diluted 1:27 and then 100μl seeded per well into a 96 well plate.
Answer:
if we dilute the solution in ratio 1:27 it means that the number of cells will decrease upto 1x105 cells . if we pour 100 microliter cell solution in one well and there are 96 such wells then the cell density of one well will be 1x104 cells because the solution will again be diluted by 1:10 ratio.
Explanation:
Diluting the cell culture 1:27 results in a solution containing 1 x 105 cells/ml, by seeding 100μl per well you are effectively diluting the solution by a further 1:10 - this means each well will contain 1 x 104 cells.