Question

The use of biotinylated secondary
antibody in ELISA increases_
of the assay without compromising the_​

Answer 1

Answer:

A secondary antibody aids in the detection, sorting or purification of target antigens by binding to the primary antibody, which directly binds to the target antigen.Advantages of using secondary antibodies

Secondary antibodies are used for the indirect detection of a target to which a specific primary antibody is first bound. The secondary antibody must have specificity both for the antibody species as well as the isotype of the primary antibody being used.The primary antibody is first incubated with the sample to allow for binding with the target antigen. Following this, a biotinylated secondary antibody is incubated along with the tissue, causing binding to the primary antibody.Biotinylated secondary antibodies

Avidin or streptavidin form strong complexes with biotin providing high signal amplification due to the large binding capacity of biotin (up to four molecules of avidin or streptavidin).

Explanation:

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