Question

The use of insulin to purify its receptor is an example of which chromatography?

Answer 1

The use of insulin hormone to purify its receptor is an example of a) Ion exchange ... c) Gel filtration chromatography d) Ligand mediated chromatography.

Answer 2

Answer: The correct answer is the use of insulin to purify its receptor is called Affinity Chromatography.

Explanation:

• According to a highly precise macromolecular binding contact between a biomolecule and another material, affinity chromatography is a technique for extracting a biomolecule from a mixture.
• Proteins are separated using affinity chromatography based on how they interact with a particular ligand. By using either competition or lowering the affinity with pH and/or ionic strength, the protein's binding to a ligand coupled to a matrix is reversed.
• Work with biological materials and pharmacological compounds now increasingly relies on the separation technique known as affinity chromatography.
• This technique relies on the employment of a stationary phase that is connected to biology in order to preferentially retain analytes or investigate biological interactions.

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