The various methods of achieving enzyme stability include-
(a) Stabilization of soluble enzymes
(b) Stabilization by cross-linkage (immobilization) of enzyme molecules or cells
(c) Bonding to carriers
(d) All of the above
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Traditionally, four methods are used for enzyme immobilization, namely (1) non-covalent adsorption and deposition, (2) physical entrapment, (3) covalent attachment, and (4) bio-conjugation (Fig. 2). Support binding can be physical or chemical, involving weak or covalent bonds.
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