The vector also have one unique recognition site to enable foreign DNA to be inserted into the vector during the generation of an rDNA molecule. Most of the commonly used vectors contains unique recognition sites region of DNA which is referred to as a polylinker or multiple cloning site (MCS). An MCS provides:
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An MCS provides:
- A polylinker or MCS is an inserted DNA fragment (often synthetic), with multiple recognition sequences for several commonly used restriction endonucleases
- MCS (polylinker) is included in plasmid vectors often via genetic engineering.
- An MCS allows scientists to clone a variety of different fragments generated by many commonly used restriction enzymes.
- They can also be used later as points for inserting additional DNA by cleavage and ligation.
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