there are two plasmids A and B are given. A has 16 recognition site for ECORi and B has 2 sites .which of them are chosen by you to insert a gene if both have the same copy number give reason for your choice what would the basic sequence for ecori
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Much effort has been spent on developing cloning vectors that replicate in organisms other than E. coli.
a. Describe several different reasons one might want to
clone DNA in an organism other than E. coli.
b. What is a shuttle vector, and why is it used?
c. Describe the salient features of a vector that could be used for cloning DNA in yeast
a. Describe several different reasons one might want to
clone DNA in an organism other than E. coli.
b. What is a shuttle vector, and why is it used?
c. Describe the salient features of a vector that could be used for cloning DNA in yeast
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