three stages by which recombinant DNA technology is carried out.
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Answered by
36
Hi.
here is your answer.
● The recombinant DNA techniques was 1st proposed by peter lobban , a graduate student , with A. dale kaiser at the department of biochemistry.
● The present day rDNA technology actually flourished after Stanley cohen and boyer (1972 ) could successfully link a genetic coding for antibiotic resistance with a native plasmid of salmonella typhimurium with the vector plasmid and then cloning it in e coli
● Recombinant DNA (rRNA ) technology is the technique of manipulating the genome of cell or organism so as to change the phenotype desirably.
●Following are the basic steps involved in the process.
● Isolating genomic DNA of a donor .The cell or organism from which the required gene is taken called donor.
● Fragmentation this DNA using molecular scissors.
● Screening the fragments of desire gene.
● Inserting the fragments with the desired gene into a cloning vector. so as to develop a recombinant dna or chimeric DNA.
●Introducing the recombinant vector into a competent host cell.
● Culturing these cells to obtain multiple copies or clones of desired fragments of DNA.
● Using this copies to transfer suitable host cells so as to express the desired gene..
hope it helps you. ☺
here is your answer.
● The recombinant DNA techniques was 1st proposed by peter lobban , a graduate student , with A. dale kaiser at the department of biochemistry.
● The present day rDNA technology actually flourished after Stanley cohen and boyer (1972 ) could successfully link a genetic coding for antibiotic resistance with a native plasmid of salmonella typhimurium with the vector plasmid and then cloning it in e coli
● Recombinant DNA (rRNA ) technology is the technique of manipulating the genome of cell or organism so as to change the phenotype desirably.
●Following are the basic steps involved in the process.
● Isolating genomic DNA of a donor .The cell or organism from which the required gene is taken called donor.
● Fragmentation this DNA using molecular scissors.
● Screening the fragments of desire gene.
● Inserting the fragments with the desired gene into a cloning vector. so as to develop a recombinant dna or chimeric DNA.
●Introducing the recombinant vector into a competent host cell.
● Culturing these cells to obtain multiple copies or clones of desired fragments of DNA.
● Using this copies to transfer suitable host cells so as to express the desired gene..
hope it helps you. ☺
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Answered by
16
Hey !!!
Here is your answer --
____________________________________
RECOMBINANT DNA TECHNOLOGY-
When two DNA molecules from different sources join together and inserted into another organism (host) to make a new product for human welfare then the process is known as Recombinant DNA Technology.
Steps --
✓ ISOLATION OF GENETIC MATERIAL - First of all desired DNA in its pure form i.e; free from another macromolecules are isolated.
✓ RESTRICTION ENZYME DIGESTION - Restriction enzymes (i.e; Restriction Endonuclease) cuts DNA at specific location therefore known as Biological Scissor.
✓ AMPLIFICATION USING PCR - PCR ( Polymerase Chain Reaction ) is a method of making multiple copies of a DNA sequence using the enzyme DNA Polymerase.
✓ LIGATION OF DNA MOLECULES - After cutting the fragment of DNA and vector by Restriction Endonuclease, their fragments are tied together by using enzyme 'DNA Ligase' is called Ligation resulting DNA is Recombinant DNA
✓ INSERTION OF RECOMBINANT DNA INTO HOST - Now the recombinant DNA is introduced into a receipt host cell. This process is called Transformation.
✓ OBTAINING FOREIGN GENE PRODUCT - After inserting the recombinant DNA, it multiplied in the host and is expressed as host. These proteins are called as Recombinant Protein.
✓ DOWNSTREAM PROCESSING - In this process the final product is subjected to downstream processed. These processes are -
Separation and purification.
Formulation with suitable preservatives.
Clinical trials to test the efficacy and safety of the product.
Quality control tests.
____________________________________
hope it will help you...
best of luck...
Here is your answer --
____________________________________
RECOMBINANT DNA TECHNOLOGY-
When two DNA molecules from different sources join together and inserted into another organism (host) to make a new product for human welfare then the process is known as Recombinant DNA Technology.
Steps --
✓ ISOLATION OF GENETIC MATERIAL - First of all desired DNA in its pure form i.e; free from another macromolecules are isolated.
✓ RESTRICTION ENZYME DIGESTION - Restriction enzymes (i.e; Restriction Endonuclease) cuts DNA at specific location therefore known as Biological Scissor.
✓ AMPLIFICATION USING PCR - PCR ( Polymerase Chain Reaction ) is a method of making multiple copies of a DNA sequence using the enzyme DNA Polymerase.
✓ LIGATION OF DNA MOLECULES - After cutting the fragment of DNA and vector by Restriction Endonuclease, their fragments are tied together by using enzyme 'DNA Ligase' is called Ligation resulting DNA is Recombinant DNA
✓ INSERTION OF RECOMBINANT DNA INTO HOST - Now the recombinant DNA is introduced into a receipt host cell. This process is called Transformation.
✓ OBTAINING FOREIGN GENE PRODUCT - After inserting the recombinant DNA, it multiplied in the host and is expressed as host. These proteins are called as Recombinant Protein.
✓ DOWNSTREAM PROCESSING - In this process the final product is subjected to downstream processed. These processes are -
Separation and purification.
Formulation with suitable preservatives.
Clinical trials to test the efficacy and safety of the product.
Quality control tests.
____________________________________
hope it will help you...
best of luck...
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