Question

Ti plasmids are achieved by

a) Vectorless DNA transfer b) Physical methods of DNA transfer

c) Chemical methods of DNA transfer d) Vector mediated gene transfer​

Answer 1

Answer:

a) Transfer of desired genes from one organism into another is an important aspect of genetic engineering. (d) Particle gun/ Biolistic method: It is a technique in which tungsten or gold particle coated with foreign DNA are bombarded into target cells to facilitate entry of the foreign genes. ...

B) Electroporation, biolistics transformation, gene gun, protoplast fusion and microinjection are some common physical gene transfer techniques used often in genetic engineering experiments

C) Chemical based methods of gene delivery can use natural or synthetic compounds to form particles that facilitate the transfer of genes into cells. These synthetic vectors have the ability to electrostatically bind DNA or RNA and compact the genetic information to accommodate larger genetic transfers.

D) Vector-mediated gene transfer is carried out either by Agrobacterium-mediated transformation or by use of plant viruses as vectors. ADVERTISEMENTS: Agrobacterium-Mediated Gene Transfer: Agrobacterium tumefaciens is a soil-borne, Gram-negative bacterium.

Explanation:

Hope it's helpful to u