To be a cloning vector, a plasmid does not require
a. An origin of replication
b. an antibiotic resistance marker
c. a restriction site
d. to have a high copy number
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Answer:
c is the correct answer
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To be a cloning vector, a plasmid does not require d. to have a high copy number.
Explanation:
- A small segment of DNA into which a desired foreign DNA fragment can be integrated for producing more copies of the desired DNA fragment is called as cloning vector.
- Cloning vectors play a major role in gene cloning process.
- Cloning vectors contain the following:
- Origin of replication
- Selectable marker gene (Eg: Antibiotic resistance marker)
- Unique restriction site
- Hence option d. to have a high copy number is not required in a plasmid.
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