Question

To clone into a plasmid vector, both the plasmid and the foreign DNA are cut
A.with the same restriction enzyme and mixed together
B. with different restriction enzyme and mixed together
C. with the combination of enzymes and then seperated
D. with the combination of enzymes and mixed together

Answer 1

Hey mate.....

here's Ur answer.....

Ques))) To clone into a plasmid vector, both the plasmid and the foreign DNA are cut....

Ans)))) with the combination of enzymes and mixed together.....

Option D....

Hope it Helps❤❤

Answer 2

Answer:

The correct option is (D). Both the plasmid DNA and foreign DNA are cut with combination of enzymes and mixed together.

Explanation:

• Making numerous, exact copies of a specific segment of DNA is known as DNA cloning. The gene or other DNA fragment of interest (perhaps a gene for a therapeutically significant human protein) is first put into a circular piece of DNA called a plasmid in a DNA cloning method.
• Recombinant DNA, or DNA that has been constructed from fragments from several sources, is the result of the insertion, which is carried out by enzymes that "cut and paste" DNA.
• Next, bacteria are given the recombinant plasmid. The plasmid-carrying bacteria are chosen and raised.
• They make copies of the plasmid's DNA during reproduction and pass those copies on to their progeny.

The correct option is (D).

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