to determine buffering ranges and capacities along with pka values for biologically relevant buffers
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Predicting the pH of a Buffer. The pH of a buffer is determined by two factors; 1) The equilibrium constant Ka of the weak acid and 2) the ratio of weak base [A-] to weak acid [HA] in solution.
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Amongst the buffers with temperature dependent pKa values, for example, are the amine buffers, whilst carboxylic acid buffers generally react less sensitively to changes in temperature. The pH value of a Tris solution set at a pH of 7.8 at room temperature is 8.4 at 0°C and 7.4 at 37°C.
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